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Rabbit Secondary Antibodies

Secondary antibodies where rabbit is the host species. Secondary antibodies must be raised against the host species of the primary antibody. Includes different antigens, classifications, conjugations, and isotypes.
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Rabbit Anti-Goat IgG (H&L) Antibody (HRP), ProSci

Secondary Antibody

Content And Storage Peroxidase Conjugated Affinity Purified anti-Goat IgG can be stored vial at 4 °C prior to restoration. Resuspend with 1.0 ml of deionized water (or equivalent. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Target Species Goat
Host Species Rabbit
Conjugate HRP
Applications ELISA,Western Blot,Immunohistochemistry
Form Lyophilized
Isotype IgG
Antigen Rabbit Anti-Goat IgG (H&L)
Regulatory Status RUO
Purification Method Peroxidase Conjugated Affinity Purified anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Dilution Peroxidase Conjugated Affinity Purified anti-Goat IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:14,000 - 1:60,000, Western Blot 1:1,000 - 1:5,000, Immunohistochemistry 1:500 - 1:2,500. (Optimal dilution should be determined by user).
Product Type Antibody
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Classification Polyclonal
Immunogen Goat IgG - whole molecule
Primary or Secondary Secondary
Test Specificity Minimum Cross reactivity to Human Serum Proteins